Monitoring of multiple sclerosis (MS) requires of molecular
markers. Recently, using TCA-precipitation/ extraction and MALDI TOF/TOF
mass-spectrometry, we identified in blood serum of multiple sclerosis (MS)
patients earlier unknown 46 kDa form of human unconventional myosin IC isoform
b (Myo1C) (Myronovskij et al., 2015).
During SDS-electrophoresis of TCA-extracted proteins of blood
serum of 28 MS patients, 3 additional polypeptides with 55, 50 and 25 kDa
molecular masses were detected. Western-blot analysis
using monospecific anti-human IgG rabbit HRP-conjugated antibodies showed
that 55 and 25 kDa polypeptides belong to heavy and light chains of IgG
molecules, while 50 kDa protein corresponds to free heavy chains of IgGs.
None of these polypeptides was found in fractions of TCA
extracted polypeptides isolated from blood serum of healthy human donors or
patients with systemic lupus erythematosis and rheumatoid arthritis.
